2006 event starts Saturday at Children\'s Hospital in Greenville. Participants are required to arrive at the hospital by 7:30 a.m. and the run begins at 8:30 Saturday morning, according to Shrine Bowl Run coach Kevin Shaw.
| Leg | Runner | Hand-Off Location | Total Miles |
| Leg 1 | Laura Eleazer | Front of Hospital, follow perimeter road to exit 2, turn right, turn left on Henrydale, turn right on Highway 29 to Cancer Society sign | 1.3 miles |
| Leg 2 | David Huckaby | Cancer Society sign to McBee V sign just over bridge | 2.6 miles |
| Leg 3 | Josh Mabry | McBee V sign just over bridge to Henry's Smokehouse | 3.9 miles |
| Leg 4 | Dusty Bateman | Henry's Smokehouse to beginning of Bob Jones university fence | 5.2 miles |
| Leg 5 | Jonathan McGinnis | beginning of Bob Jones university fence to Hampton Forest sign (on left) | 6.5 miles |
| Leg 6 | Tyler Hudgins | Hampton Forest Sign (on left) to Molina's Mexican | 7.8 miles |
| Leg 7 | Stuart McDaniel | Molina's Mexican to Spinx Gas | 9.1 miles |
| Leg 8 | Jenna Callahan | Spinx Gas to Springwell Church sign (on left) | 10.4 miles |
| Leg 9 | Travis Owens | Springwell Church Sign (on left) to Bruster's | 11.7 miles |
| Leg 10 | David Willis | Bruster's to Waffle House | 13.0 miles |
| Leg 11 | Ryan Arrowsmith | Waffle House to Pizza Hut | 14.3 miles |
| Leg 12 | Stephanie Buffo | Pizza Hut to Mr. Bingo | 15.6 miles |
| Leg 13 | Marshal Meyer | Mr. Bingo to Texaco | 16.9 miles |
| Leg 14 | Paul Bowers | Texaco to Duncan City Limit sign | 18.2 miles |
| Leg 15 | Jonathan Falco | Duncan City Limit sign to Post Office (on left @ 40 mph sign) | 19.5 miles |
| Leg 16 | Darin Mattison | Post Office (on left @ 40 mph sign) to Fred's | 20.8 miles |
| Leg 17 | Ben Miller | Fred's to Utopia Spa sign | 22.1 miles |
| Leg 18 | Stephen Usery | Utopia Spa sign to I-85 sign before overpass | 23.4 miles |
| Leg 19 | Adam Freudenthal | I-85 sign before overpass to North Tyger River sign | 24.7 miles |
| Leg 20 | Chad Holt | North Tyger River sign to A & B Aquarium | 26.0 miles |
| Leg 21 | Sam Barlow | A & B Aquarium to Target sign | 27.3 miles |
| Leg 22 | Greg Marcil | Target sign to Golden Corral (on left) | 28.6 miles |
| Leg 23 | Johnathan Chu | Golden Corral (on left) to Briarwood Road stop light | 29.9 miles |
| Leg 24 | Stephanie Gammon | Briarwood Road stop light to Fast Stop | 31.2 miles |
| Leg 25 | Josh Walker | Fast Stop to left on Church St. (Sandella's Restaurant), right on Memorial (Krispy Kreme), pass baseball field, turn left at stop sign, up hill to parking lot | 32.7 miles |